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pge2 tocris  (Tocris)


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    Tocris pge2 tocris
    Pge2 Tocris, supplied by Tocris, used in various techniques. Bioz Stars score: 96/100, based on 375 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pge2+tocris/pm41894395-233-190-191?v=Tocris
    Average 96 stars, based on 375 article reviews
    pge2 tocris - by Bioz Stars, 2026-07
    96/100 stars

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    Figure 1. A human intestinal organoid model containing BEST4/CA7+ cells (A) Schematic of organoid expansion and differentiation. Medium compositions for organoid expansion and differentiation are listed (see also STAR Methods). Representative cell types in the cultured organoids are indicated. EGF, epidermal growth factor; NAC, N-acetyl-L-cysteine; <t>PGE2,</t> prostaglandin E2; NIC, nicotinamide; RSPO1, R-spondin1. (B) qPCR analysis of a set of BEST4/CA7+ cell markers in colon organoids cultured in expansion or differentiation medium. n = 3 biological replicates. Data are presented as mean ± SD. Paired, two-tailed t test is used for comparisons. ***p < 0.001, **p < 0.01, *p < 0.05. (C and D) Representative images of colon (C) and small intestine (D) organoids cultured in differentiation medium. BEST4/CA7+ cells are marked by whole-mount immunofluorescence staining of an antibody against BEST4 (green). Scale bars, 50 mm. See also Figure S1.
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    Figure 1. A human intestinal organoid model containing BEST4/CA7+ cells (A) Schematic of organoid expansion and differentiation. Medium compositions for organoid expansion and differentiation are listed (see also STAR Methods). Representative cell types in the cultured organoids are indicated. EGF, epidermal growth factor; NAC, N-acetyl-L-cysteine; PGE2, prostaglandin E2; NIC, nicotinamide; RSPO1, R-spondin1. (B) qPCR analysis of a set of BEST4/CA7+ cell markers in colon organoids cultured in expansion or differentiation medium. n = 3 biological replicates. Data are presented as mean ± SD. Paired, two-tailed t test is used for comparisons. ***p < 0.001, **p < 0.01, *p < 0.05. (C and D) Representative images of colon (C) and small intestine (D) organoids cultured in differentiation medium. BEST4/CA7+ cells are marked by whole-mount immunofluorescence staining of an antibody against BEST4 (green). Scale bars, 50 mm. See also Figure S1.

    Journal: Cell stem cell

    Article Title: Interferon-responsive intestinal BEST4/CA7 + cells are targets of bacterial diarrheal toxins.

    doi: 10.1016/j.stem.2025.02.003

    Figure Lengend Snippet: Figure 1. A human intestinal organoid model containing BEST4/CA7+ cells (A) Schematic of organoid expansion and differentiation. Medium compositions for organoid expansion and differentiation are listed (see also STAR Methods). Representative cell types in the cultured organoids are indicated. EGF, epidermal growth factor; NAC, N-acetyl-L-cysteine; PGE2, prostaglandin E2; NIC, nicotinamide; RSPO1, R-spondin1. (B) qPCR analysis of a set of BEST4/CA7+ cell markers in colon organoids cultured in expansion or differentiation medium. n = 3 biological replicates. Data are presented as mean ± SD. Paired, two-tailed t test is used for comparisons. ***p < 0.001, **p < 0.01, *p < 0.05. (C and D) Representative images of colon (C) and small intestine (D) organoids cultured in differentiation medium. BEST4/CA7+ cells are marked by whole-mount immunofluorescence staining of an antibody against BEST4 (green). Scale bars, 50 mm. See also Figure S1.

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-BEST4 antibody (polyclonal) ATLAS antibodies Cat# HPA058564; RRID: AB_2683759 Rabbit anti-Chromogranin A (CHGA) antibody (polyclonal) Lab Ned Cat# LN1401487; RRID: N/A Rabbit anti-CA7 antibody (polyclonal) Sigma-Aldrich Cat# HPA047237; RRID: AB_2679994 Rabbit anti-GUCY2C antibody (monoclonal, clone# 2543C) R&D Systems Cat# FAB2157G; RRID: N/A Mouse anti-BrdU antibody (monoclonal, clone# MoBU-1) Thermo Fisher Cat# B35128; RRID: AB_2536432 Alexa Fluor 488 donkey anti-rabbit Thermo Fisher Cat# A21206; RRID: AB_2535792 Alexa Fluor 647 donkey anti-mouse Thermo Fisher Cat# A31571; RRID: AB_162542 Alexa Fluor 647 donkey anti-rabbit Thermo Fisher Cat# A31573; RRID: AB_2536183 Chemicals, Peptides, and Recombinant Proteins BME (cultrex Basement Membrane Extract, growth factor reduced, type 2) R&D Systems Cat# 3536-005-02 adDMEM/F12 (advanced Dulbecco’s Modified Eagle’s Medium/F12) Gibco Cat# 12634028 P/S (Penicillin/Streptomycin) Gibco Cat# 15140122 HEPES Gibco Cat# 15630056 Glutamax Gibco Cat# 35050038 B-27 supplement Thermo Fisher Cat# 12587010 NAC (N-acetyl-L-cysteine) Sigma-Aldrich Cat# A9165 Noggin conditioned medium U-Protein Express BV Custom order WNT surrogate U-Protein Express BV Custom order EGF (human Epidermal Growth Factor) Peprotech Cat# AF-100-15 A83-01 Tocris Cat# 2939 SB202190 Sigma-Aldrich Cat# S7067 PGE2 (Prostaglandin E2) Tocris Cat# 2296 NIC (Nicotinamide) Sigma-Aldrich Cat# N0636 R-spondin1 conditioned medium U-Protein Express BV Custom order IL-1b Peprotech Cat# 200-01B IL-2 Peprotech Cat# 200-02 IL-4 Peprotech Cat# 200-04 IL-6 Peprotech Cat# 200-06 IL-8 Peprotech Cat# 200-08 IL-17A Peprotech Cat# 200-17 IL-17E (IL-25) Peprotech Cat# 200-24 IL-22 Peprotech Cat# 200-22 IFN-a Peprotech Cat# 300-02AA IFN-g Peprotech Cat# 300-02 RANKL Peprotech Cat# 310-01C TNF-a Peprotech Cat# 300-01A BMP-2 Peprotech Cat# 120-02C BMP-4 Peprotech Cat# 120-05ET LPS (Lipopolysaccharides) Sigma-Aldrich Cat# L4391 Rapamycin Selleckchem Cat# S1039 DAPT Sigma-Aldrich Cat# D5942 Linaclotide Targetmol Chemicals Cat# T11852 GlyH-101 MedChemExpress Cat# HY-18336 (Continued on next page) e1 Cell Stem Cell 32, 1–15.e1–e5, April 3, 2025

    Techniques: Cell Culture, Two Tailed Test, Staining